Excellent western blot results with vertically cut strips and fluorescence detection. Comparison of mini blots processed manually (probing and washing steps performed in a tray) vs. with the iBind Flex Western Device. Blots were produced by separating samples on Bolt 4–12%, 10-well gels with MES SDS running buffer, rapid-dry transfer to nitrocellulose membrane using the iBlot 2 system, and then cutting each into three-lane strips. Final imaging was performed using a fluorescence imaging instrument.
Samples and lanes were as follows:
- Lane 1: Thermo Scientific PageRulerPrestained NIR Protein Ladder (3 μL)
- Strip 1: Phosphorylated Akt cell extract (15 μg, 7.5 μg, 3.75 μg) and Elk-1 fusion protein (150 ng, 75 ng, 37.5 ng)
- Strip 2: HeLa cell extract (30 μg, 15 μg, 7.5 μg)
- Strip 3: Phosphorylated Akt cell extract (15 μg, 7.5 μg, 3.75 μg)
- Strip 4: Elk-1 fusion protein (150 ng, 75 ng, 37.5 ng)
- Strip 5: HeLa cell extract (30 μg, 15 μg, 7.5 μg)
- Strip 6: HeLa cell extract (30 μg, 15 μg, 7.5 μg)
